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The production or modification of new antimicrobial compounds available to enhance the antibacterial activity for treatment, disinfection, or antisepsis is a highly important field of study. In this endeavor, nanotechnology is emerging as a multidisciplinary field of science in which a wide range of metal nano nanoparticles (NPs) have been synthesized. The produced NPs have a unique size with more surface area to volume ratio that promoted their reactivity with the surrounding nanoparticles include zinc oxide and silver nanoparticles; these particles have anti-microbial activity, as they have a wide surface area, which provides good connectivity to bind to their membrane cells and then penetrate into the cell, that the passage of these metal particles across the plasma membrane, bacteria lose control over the membrane permeability of incoming materials of bacteria which leads to their death Biological methods have emerged as viable options. Recently biologically-mediated synthesis of nanoparticles has been shown to be simple, cost-effective, dependable, and environmentally friendly approaches and much attention has been given to the high yield production of NPS.